First record of the complete mitochondrial genome of Culex pipiens (Diptera: Culicidae) in Iraq and its phylogenetic analysis

Authors

  • Zhuang-Xin Ye State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agricul-ture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, China
  • Adnan A. Lahuf Plant Protection Department, Agriculture College, University of Kerbala, Karbala, Iraq
  • Mohammed D. Salman Plant Protection Department, Agriculture College, University of Kerbala, Karbala, Iraq
  • Jun-Min Li State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agricul-ture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, China

DOI:

https://doi.org/10.59658/journalofkerbalaforagriculturalsciences.v10i3.1126

Keywords:

mitochondrial genome, Culex pipiens, phylogenetic analysis

Abstract

The common house mosquito, Culex pipiens, is a worldwide insect that is known as a vector of several human and animals’ pathogens that cause critical diseases, such as malaria, yellow fever and encephalitis. The mitochondrial genome knowledge can supply a foresight for evolutionary biology and phylogenetic analysis. Thus, in the current study, the complete mitochondrial genomes of the Iraqi C. pipiens were first sequenced, assembled, annotated, and analyzed using high throughput sequencing techniques. The results revealed that the length of the complete mitochondrial genome is 14,856 bp, comprising 13 protein-coding genes (PCGs), 22 transfer RNA genes, and two ribosomal RNA genes. The A + T content was 77.09%, while the G+C content was only 22.1%. All PCGs open with the start codon ATN, except the cox1 gene and end with the stop codon TAA. A phylogenetic relatedness with other different species of Culex spp. was achieved by operating the molecular evolutionary genetic analysis based on the whole mitochondrial genome sequences. The results showed that C. pipiens from Iraq shares a close ancestry with C. pipiens from Tunisia and France. To our knowledge, this is the first report of the complete mitochondrial genome sequence of the Iraqi C. pipiens. As a consequence, this conclusion may provide a new profound insight into the evolution of C. pipiens in Iraq.

Downloads

Published

03/23/2023

How to Cite

Ye, Z.-X. ., Lahuf, A. A. . ., Salman, M. D. ., & Li, J.-M. . (2023). First record of the complete mitochondrial genome of Culex pipiens (Diptera: Culicidae) in Iraq and its phylogenetic analysis . Journal of Kerbala for Agricultural Sciences, 10(3), 169–178. https://doi.org/10.59658/journalofkerbalaforagriculturalsciences.v10i3.1126

Issue

Vol. 10 No. 3 (2023): Volume 10 Issue 1 2023

Section

Articles

License

Licensing Terms

All articles are published under a Creative Commons License and will be directed to the Creative Commons Attribution Non-Commercial (CC BY NC).

The Creative Commons Attribution Non-Commercial License (CC BY NC)permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

Use by non-commercial users

For non-commercial and non-promotional purposes individual users may access, download, copy, display and redistribute the articles to colleagues, as well as adapt, translate, text- and data-mine the content subject to the following conditions:

  • The authors' moral rights are not compromised. These rights include the right of "paternity" (also known as "attribution" - the right for the author to be identified as such) and "integrity" (the right for the author not to have the work altered in such a way that the author's reputation, or integrity may be impugned).
  • Where content in the article is identified as belonging to a third party, it is the obligation of the user to ensure that any reuse complies with the copyright policies of the owner of that content.
  • If article content is copied, downloaded, or otherwise reused for non-commercial research and education purposes, a link to the appropriate bibliographic citation (authors, journal, article title, volume, issue, page numbers, DOI and the link to the definitive published version on FSP website) should be maintained.
  • Copyright notices and disclaimers must not be deleted.
  • Any translations, for which a prior translation agreement with FSP has not been agreed, must prominently display the statement: "This is an unofficial translation of an article that appeared in a FSP publication. The publisher has not endorsed this translation."

Use by commercial "for-profit" organizations

Use of FSP Open Access articles for commercial, promotional, or marketing purposes requires further explicit permission from FSP (info@fspublishers.org) and will be subject to a fee.

Commercial purposes include:

Copying or downloading of articles, or linking to such articles for further redistribution, sale or licensing; Copying, downloading or posting by a site or service that incorporates advertising with such content; The inclusion, or incorporation of article content in other works, or services (other than normal quotations with an appropriate citation) that is then available for sale or licensing, for a fee (for example, a compilation produced for marketing purposes, inclusion in a sales pack); Use of article content (other than normal quotations with appropriate citation) by for-profit organizations for promotional purposes; Linking to article content in e-mails redistributed for promotional, marketing or educational purposes; Use for the purposes of monetary reward by means of sale, resale, license, loan, transfer or other form of commercial exploitation such as marketing products; Print reprints of articles can be purchased from journal.agri@uokerbala.edu.iq.

Permissions

  • No special permission is required to reuse all, or part of article published by JKAS, including figures and tables for non-commercial purposes.
  • Any part of the article may be reused, non-commercial purposes, without permission provided that the original article is clearly cited.
  • Reuse of an article does not imply endorsement by the authors, JKAS