Extraction of two local types of cowpea (Vigna unguiculata L) proteins and study of its functional properties
Ethar Z. Naji
DOI:
https://doi.org/10.59658/jkas.v4i1.93Abstract
A study of the chemical composition of the protein isolates of two types, white and red cowpea, (obtained from the local markets in Baghdad province), which have been extracted at pH 9.5 and sedimented at 4.5, showed a higher percentage of each of the moisture, ash, protein, and low carbohydrate for red cowpea protein isolate, reached 7.12%, 2.31%, 82.21% and 8.36% compared with 6.22% , 1.57% , 79.58% and 12.63% for white cowpea protein isolate respectively, the protein solubility at different pH values ranged between 2-9, showed a lowest solubility at pH 5 while the highest at pH 9, with the values of 4.82% , 2.99% at pH 5, 23.93% , 28.24% at pH 2, and 52.15%, 51.20% at pH 9, for white and red cowpea isolates respectively, the study showed also a higher water holding capacity and lowest oil holding capacity for red cowpea protein isolate, reaching 2.05 g water/ g protein compared with 1.39 gm water/ gm protein, and 1.68, 1.53 g fat/ g protein for white and red cowpea isolates respectively. A study of the functional properties of the protein isolates at pH values 3-9, showed that a lowest were at pH 5, they were for emulsifying index 12.00% , 15. 60%, emulsion stability 15.4% , 12.6%, foaming ability 18.19% , 14.99%, and foam stability 14.89% , 12.23% for white and red cowpea protein isolate respectively, while the maximum was at pH 9 reaching, emulsifying index 80.76%, 75.41%, emulsion stability 80.9% , 90.65, foaming ability 85.43%, 83.33%, and foam stability 70.54%, 66.90% for white and red protein isolate respectively, using different concentrations ranged from 2-20 %, showed that the lowest concentration needed to form a gel was 14% of the white cowpea protein isolate, and 12% for the red one, and not configure a static gel, unless the concentration was 16% for the tow samples of the study.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2017 Journal of Kerbala for Agricultural Sciences
This work is licensed under a Creative Commons Attribution 4.0 International License.
Licensing Terms
All articles are published under a Creative Commons License and will be directed to the Creative Commons Attribution 4.0 International License That permits use, distribution, and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Use by non-commercial users
For non-commercial and non-promotional purposes individual users may access, download, copy, display, and redistribute the articles to colleagues, as well as adapt, translate, text- and data-mine the content subject to the following conditions:
- The author's moral rights are not compromised. These rights include the right of "paternity" (also known as "attribution" - the right for the author to be identified as such) and "integrity" (the right for the author not to have the work altered in such a way that the author's reputation, or integrity may be impugned).
- Where content in the article is identified as belonging to a third party, it is the obligation of the user to ensure that any reuse complies with the copyright policies of the owner of that content.
- If article content is copied, downloaded, or otherwise reused for non-commercial research and education purposes, a link to the appropriate bibliographic citation (authors, journal, article title, volume, issue, page numbers, DOI, and the link to the definitive published version on JKAS website) should be maintained.
- Copyright notices and disclaimers must not be deleted.
- Any translations, for which a prior translation agreement with JKAS has not been agreed, must prominently display the statement: "This is an unofficial translation of an article that appeared in an FSP publication. The publisher has not endorsed this translation."
Use by commercial "for-profit" organizations
Use of JKAS Open Access articles for commercial, promotional, or marketing purposes requires further explicit permission from JKAS (journal.agri@uokerbala.edu.iq) and will be subject to a fee.
The commercial purposes include:
Copying or downloading of articles, or linking to such articles for further redistribution, sale, or licensing; Copying, downloading, or posting by a site or service that incorporates advertising with such content; The inclusion, or incorporation of article content in other works, or services (other than normal quotations with an appropriate citation) that is then available for sale or licensing, for a fee (for example, a compilation produced for marketing purposes, inclusion in a sales pack); Use of article content (other than normal quotations with appropriate citation) by for-profit organizations for promotional purposes; Linking to article content in e-mails redistributed for promotional, marketing or educational purposes; Use for the purposes of monetary reward by means of sale, resale, license, loan, transfer or other form of commercial exploitation such as marketing products; Print reprints of articles can be purchased from journal.agri@uokerbala.edu.iq.
Permissions
- No special permission is required to reuse all, or part of the article published by JKAS, including figures and tables for non-commercial purposes.
- Any part of the article may be reused, for non-commercial purposes, without permission provided that the original article is cited.
- Reuse of an article does not imply endorsement by the authors, JKAS