Effect of different thawing temperature and duration of frozen seminal plasma on spermatological properties , biochemical and some enzyme of Frisian- Holstein bull.

Abstract

the study was conducted at laboratories of department of animal technical college from 1/ October /2016-1/ April /2017 to investigate the effect of thawing temperature at 30cº for 60 second and 50cº for 15 second from 0.25cc deep frozen thawed straw from main artificial insemination center Abo grabe Friesian Holstein bull aged 3-5years . On the biological properties (pH, Individual motility,  Total motility and Morphological defect )  and metabolic (total protein ,cholesterol and glucose) and enzymatic (Aspartate  and alkaline phosphatase) the result show that there is a high significant  deference (p≤0.01) at 50cº for 15 second of thawing temperature on  the alkaline phosphatase enzyme and glucose,significant  deference (p≤0.05) at 30cº for 60 second  to aspartate enzyme .