Effect of adding different levels of biotin to diet in Some blood chemo measurements are vital of broiler submitted to oxidative stress.

Abstract

This study was conducted at poultry farm /Abu- Ghraib /Dept. Animal production/ college of Agriculture/ university of Baghdad from 2/10/1016 to 12/11/2016 to study the effect of adding biotin to the diet of broiler submitted to oxidative stress on some blood chemo traits . A total of 375 , day old , unsexed Ross 308 broiler chicks was reared together until 14 day old then , distributed to five treatments with three replicates per each (25 chick.replicate^-1) . the oxidative stress was induced by adding 0.5 % H2O2 to drinking water . The treatments was as follow : T1= negative control group without adding biotin+ normal drinking water. T2= positive control group without adding biotin+ drinking water with 0.5% H₂O₂. T3= 250 µg biotin /kg diet + drinking water with 0.5% H₂O₂. T4= 350 µg biotin /kg diet + drinking water with 0.5% H₂O₂. T5= 450 µg biotin /kg diet + drinking water with 0.5% H₂O₂. Results showed that T3,T4 and T5 significantly ( p< 0.05) reduced serum glucose , ALT and AST while increased significantly ALP in comparison with T1and T2 . serum calcium didn’t affected significantly and to adding biotin while T4 and T5 increased ( p<0.05) serum phosphor glutathione ( GSH- PX) and catalase ( CAT) in comparison with T2. . We can concluded from recent study that adding biotin to broiler diet at the rat 350 and 450 mg.kg^-1 could reduce the deleterious effect of oxidative stress.