Detection of Indole Acetic Acid and Gibberelic acid in culture filtrate of local plant growth promoting fungi
DOI:
https://doi.org/10.59658/jkas.v5i5.43Keywords:
Aspergillus. Fumigatus، Aspergillus. niger، Trichoderma spp ، IAA ، GA3Abstract
The study aimed at investigating the ability of local isolates of plant growth promoting fungi: A-7 (Aspergillus fumigatus) , AD-1 (A. niger) and T-113 isolates of Trichoderma hamatum, to production of plant hormones, indole acetic acid (IAA) and Gabriellene (GA3) .The study was conducted in the laboratories of the Faculty of Agriculture - University of Kufa to evaluated ability of these fungi to produce plant hormones in their filtrate in shaking and static incubation.
It was found that three fungal A-D-1, A-7 and T-113 produced IAA and GA3 hormones in both shaking (120 r/min) and static incubation for 5, 10 and 15 days respectively at 28 +2 C. the fungus A-D-1 has a highest average for filtrate content of IAA 153.3 mg / L, followed by A-7 with 137.8 mg /L, while isolation T-113- has lowest average 90 mg / L in shaking incubation conditions. In static incubation condition, fungus A-7 has highest average of filtrate content of IAA hormone 1211 mg / L, followed by A-D-1 221 mg / L, while the isolate T-113- has lowest average with 118 mg / L.
Production of GA3 hormone identify through fungal filtrate content in both shaking 120 r/min and static incubation for 7, 14 and 21 days respectively at 28 +2 C. the fungus A-7 has a highest average of filtrate content of GA3 hormone with 2.018 mg / L, followed by A-D-1 with an average 0.742 mg / L, while isolate T-113 has lowest average 0.698 mg / L after 21 days in shaking incubation conditions. In static incubation condition, isolate A-7 has highest average of filtrate content of GA3 hormone 2.433 mg / L, followed by isolate A-D-1 0.477 mg /l, while the isolate T-113 has lowest average 0.443 mg / L.
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