Detection of Indole Acetic Acid and Gibberelic acid in culture filtrate of local plant growth promoting fungi

Abstract

    The study aimed at investigating the ability of local isolates of plant growth promoting fungi: A-7 (Aspergillus fumigatus) , AD-1 (A. niger) and  T-113 isolates of Trichoderma hamatum, to production of plant hormones, indole acetic acid (IAA) and Gabriellene (GA₃) .The study was conducted in the laboratories of the Faculty of Agriculture - University of Kufa to evaluated  ability of these fungi to produce plant hormones in their filtrate in shaking and static incubation.

    It was found that three fungal A-D-1, A-7 and T-113 produced IAA and GA₃ hormones in both shaking (120 r/min) and static incubation for 5, 10 and 15 days respectively at 28 +2 C. the fungus A-D-1 has a highest average for filtrate content of IAA 153.3 mg / L, followed by A-7 with 137.8 mg /L, while isolation T-113- has lowest average 90 mg / L in shaking incubation conditions. In static incubation condition, fungus A-7 has highest average of filtrate content of IAA hormone 1211 mg / L, followed by A-D-1 221 mg / L, while the isolate T-113- has lowest average with 118 mg / L.

    Production of GA₃ hormone identify through fungal filtrate content in both shaking 120 r/min and static incubation for 7, 14 and 21 days respectively at 28 +2 C. the fungus A-7 has a highest average of filtrate content of GA₃ hormone with 2.018 mg / L, followed by A-D-1 with an average 0.742 mg / L, while isolate T-113 has lowest average 0.698 mg / L after 21 days in shaking incubation conditions. In static incubation condition, isolate A-7 has highest average of filtrate content of GA₃ hormone 2.433 mg / L, followed by isolate A-D-1 0.477 mg /l, while the isolate T-113 has lowest average 0.443 mg / L.