Effect of Type and Concentration of Organic Acids on Soil Bacteria Growth

Authors

  • Doaa Mohammed Waheed Soil and Water Department, Agriculture College, University of Kufa, Najaf, Iraq
  • Alaa Edan Hassan Soil and Water Department, Agriculture College, University of Kufa, Najaf, Iraq

DOI:

https://doi.org/10.59658/jkas.v10i4.1299

Keywords:

Organic Acids , Enterobacter cloacae ، Bacillus Subtilis

Abstract

The study was carried out at the University of Kufa - College of Agriculture for the season  2023-2022  to study the effect of the type and concentration of organic acids on the growth of soil bacteria, the first experiment includes the cultivation of bacteria on optional, broth, four types of organic acids were used  (Citric acid, Acetic  acid, Oxalic acid, Propionic acid)  in concentrations (0,25.50.75.100.150) mM factor included four types of bacteria (Pseudomonas fluorescens, Acinetobacter calcoaceticus, Bacillus subtilis, Enterobacter cloacae). During this experiment, the minimum inhibitory concentration of these organic acids was determined, the second experiment included planting wheat seeds of a local variety by 4-2seeds per pot and used two types of organic acids (Acetic acid, Oxalic acid) and two types of pathogenic bacteria and biological resistance (Enterobacter cloacae and Bacillus subtilis).  The results of the laboratory experiment showed a significant effect of organic acids in reducing the growth of bacteria, the addition of organic acids to the soil had a significant effect on the values of (Ec, PH) in the soil as oxalic acid obtained  the highest rate of 6.370 dc m-1 At  a concentration of 50 mM, oxalic acid obtained  the highest rate of soil reaction score of 6.300 at a concentration of 75.50mM.  The results indicate the significant effect of organic acids in inhibiting the total bacteria population in the soil, as oxalic acid obtained  the highest rate of 106×114.3 g-1 dry  soil at 50mM concentration.

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Published

12/15/2023

How to Cite

Waheed, D. M. ., & Hassan, A. E. . (2023). Effect of Type and Concentration of Organic Acids on Soil Bacteria Growth. Journal of Kerbala for Agricultural Sciences, 10(4), 108–118. https://doi.org/10.59658/jkas.v10i4.1299