Biochemical Study of maize (Zea mays L.) genotypes through total seed protein by SDS - PAGE

Abstract

Maize (Zea mays L.) is one of the world's most important cereals, serving as a primary source of subsistence and industrial products. The remarkable phenotypic diversity exhibited by different maize genotypes depends on their complex biochemical compositions. In pursuit of a deeper understanding of the genetics and biochemistry of maize, this research explores the overall seed protein profiles of diverse maize genotypes using Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). Primary objectives include comprehensive analysis and comparison of total seed protein composition between distinct maize genotypes, identification of protein differences associated with phenotypic differences, and insight into the genetic and environmental determinants of maize seed protein composition. Through state-of-the-art analytical techniques, including SDS-PAGE, This research examined protein supplements found in corn seeds. The results promise to reveal the genetic and environmental factors that shape the biochemistry of corn. The patterns of the total protein gave a number of 12 main bands, whose weights ranged between 17 kDa and 245 kDa. The total number of bands reached 108 bands, including 8 homogeneous bands and 6 heterogeneous bands. . The percentage of variation (difference) between the compositions was 32%, which indicates that the percentage of similarity (similarity) was 68%. The level of similarity is higher than the level of dissimilarity between the studied structures and this is due to the geographical origin of the structures and their genetic material, or that the plant is the result of hybridization between species, which ultimately benefits humanity’s quest to achieve sustainable agriculture and improved nutrition.