Detection of the Genetic Stability for the Tissue Digitalis lanata Plants that Seeds Irradiation with Gamma Ray by Using RAPD Technique

Authors

  • Sarab A. Almukhtar College of Agriculture – Kerbala University
  • Zaid K. Kahdim College of Agriculture – Kerbala University
  • Aymen J. Mahdi College of pharmacy – Kerbala University

DOI:

https://doi.org/10.59658/jkas.v5i1.149

Keywords:

Digitalis lanata, genetic stability, RAPD technique, gamma ray, in vitro

Abstract

Randomized Amplified Polymorphic DNA (RAPD) technique was used to validate the genetic stability of the digitalis plantlets produced by irradiated seeds by (0, 10, 20, 30) doses of gamma irradiation to stimulate their germination and growth. The irradiated seed was planted on the growth regulator-free MS medium. DNA print tests were performed by using RAPD markers. DNA was extracted from the green leaf samples of the tissue culture plants for each treatment with control at 1 month age. After determining the optimal conditions for RAPD reactions, usingeight starters OPA11, OPA7, OPA7, OPA7, OPC2, OPA11, OPA2 and OP511, with a perfect match in the distribution pattern of the packages for the irradiated treatments. RAPD markers proved to be an easy and rapid DNA marker for genetic compatibility. Therefore, the study proved that the doses used are catalysts and this is the purpose of their use, and there was no genetic heterogeneity in the irradiated seeds or the resulting plants compared with the treatment of non-irradiated control.

Author Biographies

Sarab A. Almukhtar, College of Agriculture – Kerbala University

Lecturer

Zaid K. Kahdim, College of Agriculture – Kerbala University

Assistant Lecturer

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Published

03/29/2018

How to Cite

Almukhtar, S. A., Kahdim, Z. K., & Mahdi, A. J. (2018). Detection of the Genetic Stability for the Tissue Digitalis lanata Plants that Seeds Irradiation with Gamma Ray by Using RAPD Technique. Journal of Kerbala for Agricultural Sciences, 5(1), 65–80. https://doi.org/10.59658/jkas.v5i1.149

Issue

Vol. 5 No. 1 (2018): Vo.5 No.1 2018

Section

Previous publications

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Copyright (c) 2018 Copyright (c) 2024 is the Author's article. Published by the Journal of Kerbala for Agricultural Sciences under a CC BY 4.0 license

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