The effect of an induced hypercholesteremia on the regulation of the ARC gene expression, Brain Derived Neurotropic Factor (BDNF), Synapsing-1 and neurotransmitter –glutamate in male rats
DOI:
https://doi.org/10.59658/jkas.v11i2.1842Keywords:
hypercholesteremic diet, glutamate, BDNF, oxidative stress.Abstract
An abnormality of lipid metabolism known as hypercholesteremia, which causes blood cholesterol levels to be abnormally high, is a significant health concern. This research, in light of its association with neurodegenerative disease, is of paramount importance as it aims to elucidate how elevated cholesterol levels impact glutamate levels, BDNF, Synapsin K, and ARC gene expression. Ten male rats were meticulously assigned to a control group and another group that was given a 1% cholesterol supplement for 28 days. Each group received a random assignment of rats to ensure the validity of the results. Samples of homogenized brain tissue and serum blood were obtained after the experiment's completion, following a meticulous experimental design. Triacylglycerol (TAG), low-density lipoprotein (LDL), and total cholesterol (TC) values rose significantly, whereas HDL and brain-derived neurotrophic factor (BDNF) fell sharply, according to the study's findings. The results indicated that hypercholesteremia had a negative impact on glutamate levels, BDNF, and synapses due to oxidative stress, while synapsin-1 levels in brain tissue remained unchanged. There were also notable decreases in serum and homogenized brain tissue concentrations of NO and GSH, along with increases in MDA. Additionally, there was a noticeable decrease in glutamate levels in brain tissue and down-regulation of the Activity Cytoskeleton Association protein gene (ARC gene) expression.
Downloads
Published
How to Cite
License
Copyright (c) 2024 Copyright (c) 2024 is the Author's article. Published by the Journal of Kerbala for Agricultural Sciences under a CC BY 4.0 license
This work is licensed under a Creative Commons Attribution 4.0 International License.
Licensing Terms
All articles are published under a Creative Commons License and will be directed to the Creative Commons Attribution 4.0 International License (CC BY 4.0) That permits use, distribution, and reproduction in any medium, provided the original work is properly cited. This license also allows the work to be used for commercial purposes.
Use by both non-commercial and commercial users
This content is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) license, permitting use by both non-commercial and commercial users. Individual users may access, download, copy, display, and redistribute the articles to colleagues, as well as adapt, translate, and text- and data-mine the content, subject to the following conditions:
- The author's moral rights, including the right of attribution and the right to protect their work from derogatory treatment, are respected.
- Where content in the article is identified as belonging to a third party, users must ensure that any reuse complies with the copyright policies of the owner of that content.
- If the article content is reused for research or educational purposes, users should maintain a link to the appropriate bibliographic citation, including the DOI and a link to the published version on the journal's website.
