Different Isolate Comparison of Alternaria alternata in their pathogenisity ability and molecular Diagnosing
DOI:
https://doi.org/10.59658/jkas.v2i3.505Abstract
Series of Laboratory experiment were conducted at the Department of plant protection ,College of Agriculture ,University of Kerbala. Fours fungal isolate designated here after by AM1 , AM2 , AB and AK of Alternaria alternata were studied . show that more pathogenic isolate was AM2 , germination percentage of the cress seeds and tomatoes was 0.0% for both , inhibition percentage was 100%. Then , in A.B isolate, germination percentage of the cress seeds was 4.4% and tomatoes 0.0% and inhibition percentage was 49.97% , 100% respectively. Then ,in A.M1 isolate, germination percentage of the cress seeds and tomatoes were 8.86% and 73.33% respectively, while the inhibition percentages of the cress seeds and tomatoes were 89.87% 22.9% respectively. The less pathogenic isolate was AK , the germination percentages of the cress seeds and tomatoes were 60.0% and 83.66% respectively and the inhibition percentages of the cress seeds and tomatoes were 31.42% and 12.12% respectively. where other isolate were less pathogenitic on the seed .these isolates were diagnosed by using PCR technique which were molecularly similar.
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