Effect of some biological agents and chemical fungicides to induce systemic resistance in cucumber plants against Fusarium oxysporum f.sp. cucumerinum

Abstract

          The aim of the study was to evaluate  the efficiency of some biological agents and chemical fungicides to reduce disease percentage and disease severity of Fusarium wilt disease in cucumber, which caused by Fusarium oxysporum f.sp. cucumerinum under plastic house conditions, and detection of the effectiveness of factors induction through the investigation for activity peroxidase enzyme , the amount of phenols as well as the amount of total chlorophyll in cucumber plants leaves . Results showed superiority treatments of biological agents Trichoderma viride , Trichoderma harzianum , bio-preparation Biohealth and preparation of bacterial  Fluramel (alone or in mixture). Treatments reduced disease percentage and disease severity to 0 and 0 % respectively, compared to control treatment (pathogen) was 86.67 and 68% respectively. Results showed  that after 7and 14 days of the addition pathogenic fungus to cucumber plants , was superior treatment of interaction between Biohealth with  chemical fungicide (Uniform) in the presence of pathogen in the increase activity of peroxidase enzyme where amounted to average absorption 7.17 and 5.89 min/gfw respectively . Also the same treatment superior in recording highest phenols in leaves cucumber plants after 7and 14 days of the addition treatment  pathogenic fungus to cucumber plants to 7.77 and 7.38 mg/gfw respectively, while where amounted to treatment of pathogen  2.63 and 2.48 mg/gfw respectively , and superior treatment of Biohealth on the other treatments in increase total chlorophyll after 7and 14 days of treatment with pathogenic fungus to cucumber plants to 38.3 and 36.5 Spad respectively, while in treatment of pathogenic fungus recorded 27.7 and 24.4 Spad respectively.