Effect of garlic supplementation on the humoral and cellular immunity after immunization of broilers by sonicated oocysts of Eimeria tenella

Abstract

This study was performed to investigate the effect of garlic with sonicated oocysts immunization of E. tenella on humoral and cellular immune responses in broilers. A total of 150 broiler chicks were divided into 6 equal groups. The 1^st and 2^nd groups inoculated in ovo with sonicated oosycts of E. tenella and repeated by I/M injection at day 14 .The 3^rd and 4^th groups injected I/M with sonicated oosycts at day old and repeated at day 14, the 1^st and 3^rd feeding a basal diet, while the 2^nd, 4^th and 5^th groups feeding basal diet with garlic. The birds of the 6^th group fed basal diet and remains as control. All groups were challenged with 50,000 of sporulated oocysts of E. tenella. The results revealed that the immunized groups and immunized with garlic have a significant difference P < 0.05 in the lymphoid organs weight and indices, antibody titers against SRBCs (sheep red blood cells)  and Phytohaemagglutinin (PHA) skin test in camper with control and garlic groups specially at day 35. At these instants there was no significance difference between the two procedures of immunization in ovo and I/M.

In conclusion that in ovo and I/M immuinzation of sonicated oocyst showed some evidence of reducing negative performance impact associated with coccidiosis.