MIP-1α Is More Sensitive than MCP-1, and CXCL10 Immune Markers in Diagnosis of Pediatric Sepsis

Abstract

Background: Pediatric sepsis is a leading cause of mortality among children both in Iraq and globally. The underlying causes of pediatric sepsis are diverse, encompassing a broad range of pathogenic mechanisms and varying responses to these triggers. However, a common characteristic of pediatric sepsis is the secretion of cytokines and chemokines.

Objectives: To evaluate the sensitivity and specificity of macrophage inflammatory protein-1 alpha (MIP-1α) in a comparison with monocyte chemotactic protein-1 (MCP-1) and C-X-C motif chemokine ligand 10 (CXCL10) immune markers in the pediatric sepsis and effort an evidence-based linking between the three biomarker and the laboratory value of neutrophil, lymphocyte and CRP.

Patients and Methods: This case control study includes 100 participants (50 patients and 50 control). Sandwich ELISA test used to evaluate the MIP-1α, MCP-1, and CXCL10 immune markers in pediatric sepsis and control groups.

Results: Present study shows high level of the neutrophil (62.45%), lymphocyte (47.90%) and CRP (50.52 mg / L) in the cases of pediatric sepsis compared with the normal range. Furthermore, this study shows that MIP-1α is more sensitive (82%) than MCP-1, and CXCL10 (56% and 54% respectively).

Conclusions:  At the end, this study suggests that MIP-1α has the highest diagnostic sensitivity value, so it could be concerned as essential immunodiagnostic marker for pediatric sepsis.