Effect of Modified Nanoparticles on the Levels of Some Important Proteins in Blood

Abstract

Manganese dioxide nanoparticles (MnO₂-NPs) and graphene oxide–based nanocomposites have attracted increasing attention for protein recovery applications. In this study, follicle-stimulating hormone (FSH) and luteinizing hormone (LH) were investigated as model glycoproteins for adsorption onto chitosan-coated MnO₂ nanoparticles (MnO₂-NPs) and chitosan/graphene oxide–MnO₂ nanocomposites (CS/GO-MnO₂-NPs). The CS/GO-MnO₂ nanocomposite was synthesized using a co-precipitation method and characterized by SEM, EDS, FT-IR, XRD, and particle size analysis. Adsorption experiments were conducted using fixed nanoparticle doses and varying hormone concentrations, and thermodynamic parameters were evaluated at different temperatures. The CS/GO-MnO₂-NPs exhibited high adsorption capacity for both hormones, following the Freundlich isotherm, indicating surface heterogeneity. Thermodynamic analysis confirmed that the adsorption process was spontaneous and exothermic. These findings suggest that MnO₂-based nanocomposites are promising platforms for hormone extraction and analytical applications involving human serum.