Detection of Selected Virulence Genes in Escherichia coli and Evaluation of the Antibacterial Efficacy of a Nano-Hybrid Fosfomycin in Urinary Tract Infections.

Abstract

Escherichia coli are considered as the most common microorganism in pathological samples related to urinary tract infections (UTIs) especially in women and children. They contains many virulence factors helping in pathogenicity. However they are sensitive to fosfomycin that has broad spectrum in its activity against bacteria which its activity also could be confirmed using nanobiotechnology.
This study tried to evaluate the effect of nano-prepared fosfomycin against Escherichia coli isolated from urinary tract infections and compare its efficacy with that of free fosfomycin. The formation of a new complex during the hybridization process was documented by FT-IR, AFM and XRD
Escherichia. coli isolates under study were virulent due to the possessing some of virulence genes such as afa, pap and fimh. By using nanotechnology, Fosfomycin can be used to prepare nanohybrid antibiotic by loading on Magnesium oxide. The prepared nano-particle was affective against the E. coli isolates of UTIs in one third of its concentration Therefore, the hybridization process is economically feasible.
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