Login or Register to make a submission.

Submission Preparation Checklist

As part of the submission process, authors are required to check off their submission's compliance with all of the following items, and submissions may be returned to authors that do not adhere to these guidelines.
  • The journal accepts original un published papers in different agricultural fields.
  • The submission file is in OpenOffice, Microsoft Word, or RTF document file format.
  • Where available, URLs for the references have been provided.
  • The text is single-spaced; uses a 12-point font; employs italics, rather than underlining (except with URL addresses); and all illustrations, figures, and tables are placed within the text at the appropriate points, rather than at the end.
  • The text adheres to the stylistic and bibliographic requirements outlined in the Author Guidelines.
  • Articles should be examined by academic referees, Author must correction and comments made by the referees for acceptance.
  • The papers can be written and published either in English and the author (s) must submit to the Journal website, after registering and obtaining a username and password.
  • Use Simplified Arabic line for Arabic language and Time New Roman line for English language are used , both of them should be with font size 14 for the text, 16 for the title with a single space between lines.
  • Papers should include Arabic &English abstracts and shouldn't be more than 250 words.
  • Papers arrangement is as follows: Abstract in Arabic , Abstract in English language, Introduction, Materials and Methods, Results and discussion, conclusions acknowledgement (if found) and the English References .
  • References should be listed according to the number of the reference in the text .
  • Tables &Figures should be clear and contain subtitles describing their contents .
  • Manuscript title should include the author′s addresses, their scientific titles, physical e-mail address and keywords.
  • Manuscript mustn't be more than 21 pages with 2 cm left on each side of the paper.
  • The paper manuscript must be checked for plagiarism that should not exceed 20% .

Author Guidelines

 

Identification by PCR of Fusarium culmorum Strains Producing Large and Small Amounts of Deoxynivalenol

Bakan B. Mark1         Giraud C. Delville 2             Pinson L. Torrance2

                     Professor                     Professor                  Assistant Professor

1Plant protection Department, College of Agriculture, Kangwon University, Korea.

2Plant protection Department, College of Agriculture, Friedrich-Wilhelms Germany.

Corresponding author: aqlabedy@yahoo.co.uk

Abstract:

Thirty deoxynivalenol-producing F. culmorum strains, isolated from wheat grains, were incubated in vitro and analyzed for trichothecene production.Seventeen strains produced more than 1 ppm of deoxynivalenol…..etc.

Key words: DNA barcode, COX 1 gene, ITS rDNA, fungal identification, biodiversity.

Introduction:

Trichothecenes, including deoxynivalenol, acetyldeoxynivalenol, nivalenol, and fusarenone X, are sesquiterpene toxins produced by Fusarium species, including Fusarium culmorum, which are common fungal contaminants of cereals. Trichothecenes can be found naturally worldwide on cereals (1, 2, 3), and the consumption of these toxins is a potential problem for humans and farm animals (4).

Materials and methods

strains isolated from cereals from different areas in France were used in this study, as presented in Table 1. Fusarium strains may also be obtained from the first author.

Toxin production.

Toxin production by the Fusarium strains was conducted on autoclaved wheat grains. Wheat grains (Soissons) were moistened with sterile distilled water for 4 days at 4°C until thermodynamic water activity was maximal…..etc.

Trichothecene analysis.

Wheat grains (25 g) were analyzed by gas chromatography- electron capture detection and gas chromatography-mass spectrometry etc.

Results

  1. culmorum identification. The Fusarium strains studied were isolated from commercial wheat kernels. Morphological identification of F. culmorum strains was confirmed by PCR….etc.

 

Figure 1:   nucleotide sequence generated from PCR products amplified from ……

 

Table 1: Oligonucleotide pairs used for gene specific amplification

Gene name

 

Primer

abbreviations

Primer sequence (59–39)

Amplification product

 

expected size (kb)

Isocitrate lyase*

ICL-F

GGC TGG CAG TCN TCY TCT ACM G

1

 

ICL-R

TKG TAN CGG TAG TAN CCC TCR

Transcription factor

MST12-F

GCS CCW GTN GAC TGG CAA CCC

1.1

 

MST12-R

CCS GTC TCC TCG TTG GCN ATG TA

 

 

……etc….

 

 

*Gene name and primer sequence were obtained from NCBI.

References:

1.Ahmad, T. (2001) Molecular detection and characterization of Fusarium verticillioides in maize (Zea mays. L) grown. M.Sc. Thesis, Plant Protection Dept., Coll. of Agric., Univ. of Kerbala, pp. 85. (An example of a thesis or report).

2.Agrios G. N. (2005) Plant Pathology, 4th Edition. Elsevier Academic Press, Burlingto, pp. 922. (An example of a book).

3.Godoy, P.; Cano, J.; Gene, J.; Guarro, J. Hoüfling-Lima, A. L. and Colombo, A. L. (2014) Genotyping of 44 isolates of Fusarium solani, the main agent of fungal keratitis in Brazil. Journal of Clinical Microbiology, 42: 4494-4497. (An example of a paper of a Journal).

 

 

 

Notes for the researcher(s):

  1. The standard unit writing system must be standardized as given here in this example: 100 mg. L-1.
  2. The paper will be returned to the researcher(s) if the researcher(s) does not comply with the publication instructions in our journal, and not follow all the amendments fixed by the reviewers.
  3. The editor-in-chief and members of the editorial board are entitled to refuse the paper without returning to the reviewers in case of the publishing conditions in the paper submitted to our Journal are not available.