Identification and isolation of Campylobacter jejuni and C. upsaliensis from bovine local milk, milk products, and human stool samples by molecular technique in Karbala province

Authors

  • Sara Haider Hassan Alsafar Department of Public Health, Veterinary Medicine College , University of Krbala Kerbala, Iraq .
  • Ali Ridha Abd Department of Public Health, Veterinary Medicine College , University of Krbala Kerbala, Iraq .
  • Ihab Ghazi Mahdi Department of Internal and Preventive Medicine , Veterinary Medicine College, University of Kerbala, Kerbala, Iraq.
  • Tuqah Talib Abdulazeez Al-tameemi Departement of Physiotherapy, Health and Medical Assistant Collage, University of Al-Zahra For Woman , Kerbala, Iraq.

DOI:

https://doi.org/10.59658/jkas.v10i4.1289

Keywords:

Campylobacter jejuni and C. upsaliensis, infection rate, Antimi-crobial susceptibility, Karbala retail points

Abstract

This study aimed to determine the prevalence of Campylobacter species in milk and its product samples in Karbala province and detect the antibiotic sensitivity of these isolates. One hundred samples were aseptically collected as follows: 50 samples of bovine raw milk and 50 samples of bovine milk products from different local shops and farms distributed in Karbala province, in addition to, 100 samples from children suffering diarrhea, fever, and abdominal pain from Kerbala (Iraq) General Children's Hospital. The pathogen was found using biochemical testing after samples were immediately inoculated into enriched and sub-cultured on selected media. Campylobacter spp. At 24 to 48 hours, colonies on the plate are small, often grayish in appearance, although some have to be creamy grey, slightly raised and moist, and often generated separate colonies of bacteria, flat with irregular borders, and non-hemolytic. This was confirmed by using the polymerase chain reaction method, 11 isolates (5.5%) of 200 samples were determined to be Campylobacter species. The 11 isolates were subjected to sequencing to detect the species of Campylobacter which found two species (C. jejuni and C. upsaliensis). Results showed that Campylobacter spp. 11 positive isolates, C. jejuni recorded 9 (81,8%) positive results while C. upsaliensis recorded 2 (18.18%) positive results. Antibiotic_ susceptibility_ test was cultured by using disk diffusion assay, campylobacter spp. showed complete sensitive  to ciprofloxaci , gentamycin, and Imipenem.

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Published

12/15/2023

How to Cite

Alsafar, S. H. H. ., Abd, A. R. ., Mahdi, I. G. ., & Al-tameemi, T. T. A. . (2023). Identification and isolation of Campylobacter jejuni and C. upsaliensis from bovine local milk, milk products, and human stool samples by molecular technique in Karbala province. Journal of Kerbala for Agricultural Sciences, 10(4), 1–17. https://doi.org/10.59658/jkas.v10i4.1289

Issue

Vol. 10 No. 4 (2023): volume 10 issue 4 year 2023

Section

Articles

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