Study the efficiency of three different methods for purification of k-casein from local goat milk

Abstract

      In this paper we study the efficiency of three different methods which were gel filtration chromatography, electrostatic affinity chromatography and Zittle and Custer method For the purification of k-casein from the milk of Iraqi local goats.

      Gel filtration chromatography and electrostatic affinity chromatography were the best methods for the purification of k-casein from goat's milk casein, while Zittle and Custer method was inefficient  in k-casein purification. Sialic acid concentration in goats k – casein purified by gel filtration, electrostatic affinity chromatography and Zittle and Custer method were 4.33, 4.20, 1.12 mg/g respectively.