Detection of Tomato bushy stunt virus in tomato felids by biology and serology assay, and transmit it by soil
حميد حمود علي نور صلاح احمد
DOI:
https://doi.org/10.59658/jkas.v5i5.51Keywords:
فيروس التقزم الشجيري للطماطة، التشخيص الحيوي والمصلي، نقل الفايروس بالتربةAbstract
This study was conducted out to detected the tomato bushy stunt virus in tomato felids at Ninawa Province included Salamia & Sad Badysh locations. The virus was isolated from infected tomato plants, and identified by mean symptoms on indicator plants, and DAS-ELISA test. Ch. amaranticolor was responded to inoculation by virus necrotic local lesion, D. stramonium by small chlorotic local lesions on inoculated leaves, developed later to mosaic. C. annuum by chlorotic local lesions. P. hybrid by systemic mosaic. Tobacco plants N. glutinosa , N. tabacum, N. rustica were responded to necrotic local lesion. On the other hand N. clevelandii showed a systemic mosaic with the necrosis on the top of new leaves. It was observed that the movement of TBSV from TBSV amended soil in healthy tomato seedling cultivar Jessie fast on in plants sown directly into TBSV-contaminated soil, the virus was detected by DAS-ELISA at 32 days after sowing. In seedlings, previously soon in autoclaved soil and later transplanted in TBSV-contaminated soil, TBSV was first detected at 51 days after sown. At the final harvest (110 d.a.s.) 54% of plant sown directly into TBSV-contaminated soil were found to be infected with TBSV as compared with 30% of those transplanted
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