Molecular characterization of Tomato yellow leaf curl (TYLCV) and its vector (Bemisia tabaci)
DOI:
https://doi.org/10.59658/jkas.v5i2.95Abstract
The aim of this study was to isolate and diagnose two different isolates of Tomato yellow leaf curl virus (TYLCV) and to investigate the biotype of whiteflies insects (Bemisia tabaci) using the polymerase chain reaction technique to determine the sequences of the nitrogen bases of the PCR-amplified products to identify the genetic similarity and differences between the virus isolates as well as among the biotypes of whiteflies (B. tabaci) spread in the sampling areas in some of the provinces of Iraq (Najaf, Karbala, Babylon and Dewanyia) in addition to know which of them are more prevalent in those areas.
Results of the polymerase chain reaction (PCR) test using the TY1 and TY2 primers showed the possibility of amplifying a PCR product of 580 base pairs (bp) from the TYLCV isolates obtained from some areas in the provinces of Najaf, Babylon. It was also found from the results of the analysis of the mitochondrial cytochrome oxidize I (mtCOI) sequences generated from the PCR-amplified products amplified from B. tabaci samples collected from different regions in Najaf, Karbala, Babylon and Diwaniya governorates, that the biotype B is the only biotype identified in this study, which makes the presence and dominance of this biotype more dangerous in the spread of TYLCV in nature for being one of the two biotypes of this insect that is responsible for the transmission of TYLCV.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2018 Journal of Kerbala for Agricultural Sciences
This work is licensed under a Creative Commons Attribution 4.0 International License.
Licensing Terms
All articles are published under a Creative Commons License and will be directed to the Creative Commons Attribution 4.0 International License That permits use, distribution, and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Use by non-commercial users
For non-commercial and non-promotional purposes individual users may access, download, copy, display, and redistribute the articles to colleagues, as well as adapt, translate, text- and data-mine the content subject to the following conditions:
- The author's moral rights are not compromised. These rights include the right of "paternity" (also known as "attribution" - the right for the author to be identified as such) and "integrity" (the right for the author not to have the work altered in such a way that the author's reputation, or integrity may be impugned).
- Where content in the article is identified as belonging to a third party, it is the obligation of the user to ensure that any reuse complies with the copyright policies of the owner of that content.
- If article content is copied, downloaded, or otherwise reused for non-commercial research and education purposes, a link to the appropriate bibliographic citation (authors, journal, article title, volume, issue, page numbers, DOI, and the link to the definitive published version on JKAS website) should be maintained.
- Copyright notices and disclaimers must not be deleted.
- Any translations, for which a prior translation agreement with JKAS has not been agreed, must prominently display the statement: "This is an unofficial translation of an article that appeared in an FSP publication. The publisher has not endorsed this translation."
Use by commercial "for-profit" organizations
Use of JKAS Open Access articles for commercial, promotional, or marketing purposes requires further explicit permission from JKAS (journal.agri@uokerbala.edu.iq) and will be subject to a fee.
The commercial purposes include:
Copying or downloading of articles, or linking to such articles for further redistribution, sale, or licensing; Copying, downloading, or posting by a site or service that incorporates advertising with such content; The inclusion, or incorporation of article content in other works, or services (other than normal quotations with an appropriate citation) that is then available for sale or licensing, for a fee (for example, a compilation produced for marketing purposes, inclusion in a sales pack); Use of article content (other than normal quotations with appropriate citation) by for-profit organizations for promotional purposes; Linking to article content in e-mails redistributed for promotional, marketing or educational purposes; Use for the purposes of monetary reward by means of sale, resale, license, loan, transfer or other form of commercial exploitation such as marketing products; Print reprints of articles can be purchased from journal.agri@uokerbala.edu.iq.
Permissions
- No special permission is required to reuse all, or part of the article published by JKAS, including figures and tables for non-commercial purposes.
- Any part of the article may be reused, for non-commercial purposes, without permission provided that the original article is cited.
- Reuse of an article does not imply endorsement by the authors, JKAS