Chromatographic separation and identification of fatty acids and phenolic compounds from the seeds of Citrullus colocynthis L. schrad plant growing in Iraq

Authors

  • Safaa M. Bilal Department of Biology, College of Education for Girls University of Mosul, Mosul, Iraq
  • Ayad C. Khorsheed Department of Biology, College of Education for Girls University of Mosul, Mosul, Iraq

DOI:

https://doi.org/10.59658/jkas.v9i3.996

Keywords:

Citrullus colocynthis (L.) Schrad, fatty acids, acid hydrolysis, phenolic compounds, saponification

Abstract

The current  research was presented the phytochemical composition of Citrullus colocynthis schrad seed extracts  and it was  aimed of the separation and identification of fatty acids from this plant by using a continuous soxhlet apparatus and sequence solvent systems depending on the polarity , the extracts hexane (CI1) ,chIoroform (CI2), ethyl acetate (CI3), ethanol (CI4) and hot aqueous (CI5) were obtained from this seeds and saponification process was done to obtain the free  fatty acid compounds ( palmatic, stearic, oleic, linoleic and elaidic acids ). The increasing of concentrations of fatty acid compounds was appeared from CI5 to CI1 because of decreasing of the polarity. Also, the extracts (CI3, CI4 and CI5) were carried out by the acid hydrolysis process to get the free phenolic compounds, which were identified by HPLC technique. The phenolic compounds, which were appeared in C. colocynthis Schrad seed extracts (Rutin , Caffeic acid , Ellagic acid , Gallic acid , Quercetin , Myricetin , Luteolin). Rutin and ellagic acid were appeared in the extracts ( CI3,CI4 and CI5) .Also Caffeic acid and Gallic acid was identified in CI3 and CI4 , while Quercetin and Myricetin were showed in CI4 and CI5. Finally Luteolin was only appeared in CI5.

Downloads

Published

09/12/2022

How to Cite

Bilal, S. M. ., & Khorsheed, A. C. (2022). Chromatographic separation and identification of fatty acids and phenolic compounds from the seeds of Citrullus colocynthis L. schrad plant growing in Iraq. Journal of Kerbala for Agricultural Sciences, 9(3), 59–70. https://doi.org/10.59658/jkas.v9i3.996

Issue

Vol. 9 No. 3 (2022): Volume 9 Issue 3 2022

Section

Articles

License

Licensing Terms

All articles are published under a Creative Commons License and will be directed to the Creative Commons Attribution Non-Commercial (CC BY NC).

The Creative Commons Attribution Non-Commercial License (CC BY NC)permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

Use by non-commercial users

For non-commercial and non-promotional purposes individual users may access, download, copy, display and redistribute the articles to colleagues, as well as adapt, translate, text- and data-mine the content subject to the following conditions:

  • The authors' moral rights are not compromised. These rights include the right of "paternity" (also known as "attribution" - the right for the author to be identified as such) and "integrity" (the right for the author not to have the work altered in such a way that the author's reputation, or integrity may be impugned).
  • Where content in the article is identified as belonging to a third party, it is the obligation of the user to ensure that any reuse complies with the copyright policies of the owner of that content.
  • If article content is copied, downloaded, or otherwise reused for non-commercial research and education purposes, a link to the appropriate bibliographic citation (authors, journal, article title, volume, issue, page numbers, DOI and the link to the definitive published version on FSP website) should be maintained.
  • Copyright notices and disclaimers must not be deleted.
  • Any translations, for which a prior translation agreement with FSP has not been agreed, must prominently display the statement: "This is an unofficial translation of an article that appeared in a FSP publication. The publisher has not endorsed this translation."

Use by commercial "for-profit" organizations

Use of FSP Open Access articles for commercial, promotional, or marketing purposes requires further explicit permission from FSP (info@fspublishers.org) and will be subject to a fee.

Commercial purposes include:

Copying or downloading of articles, or linking to such articles for further redistribution, sale or licensing; Copying, downloading or posting by a site or service that incorporates advertising with such content; The inclusion, or incorporation of article content in other works, or services (other than normal quotations with an appropriate citation) that is then available for sale or licensing, for a fee (for example, a compilation produced for marketing purposes, inclusion in a sales pack); Use of article content (other than normal quotations with appropriate citation) by for-profit organizations for promotional purposes; Linking to article content in e-mails redistributed for promotional, marketing or educational purposes; Use for the purposes of monetary reward by means of sale, resale, license, loan, transfer or other form of commercial exploitation such as marketing products; Print reprints of articles can be purchased from journal.agri@uokerbala.edu.iq.

Permissions

  • No special permission is required to reuse all, or part of article published by JKAS, including figures and tables for non-commercial purposes.
  • Any part of the article may be reused, non-commercial purposes, without permission provided that the original article is clearly cited.
  • Reuse of an article does not imply endorsement by the authors, JKAS