Detection of TAFI 1040C>T rs1926447 Gene Polymorphism in Type 2 Diabetes Mellitus Iraqi Patients

Abstract

In Diabetes mellitus is a group of metabolic diseases characterized by hyperglycemia resulting from defects in insulin secretion, insulin action, or both. The chronic hyperglycemia of diabetes is associated with long-term damage. Thrombin-Activatable Fibrinolysis Inhibitor(TAFI) is a protein that plays a major role in regulating the analysis of blood clots, It is involved in the fibrinolysis process. The study was designed to study the change concentration of TAFI, Insulin, HOMA-IR, and lipid profile in the T2DM patient in addition to studying  the TAFI 1040C>T SNP (rs1926447) gene polymorphism with the development of T2DM complication. Genome study doing by restriction fragment length polymorphism PCR (RFLP-PCR). These parameters included the evaluated of glucose metabolic characteristics (FSG, insulin, HbA1C, HOMA-IR, lipid profile). The expected frequencies, calculated under the assumption of no association, were 47.61for CC, 60.03, CT, and 9.61for TT. TC genotype does not appear a statistically significant linkage to the rise risk of thrombosis, OR(3.272) is and CI (0.4173 to 1.4498), p-value <0.0002 was associated with an increased danger of the thrombosis to three times. frequency of C allele among cases is 43.5%, but it is 69% among controls. Frequency T allele was among cases is 56.5%, whereas it is 31%  among controls. The findings of this investigation revealed that there was significant link between the TAFI 1040C>T polymorphism and T2DM complication in Iraqi patients. However, we recommend conducting the study in a larger sample and different sexes to confirm our findings.