The effect of an induced hypercholesteremia on the regulation of the ARC gene expression, Brain Derived Neurotropic Factor (BDNF), Synapsing-1 and neurotransmitter –glutamate in male rats

Authors

  • Hadeel Ganai Rafaish Physiology Department, Veterinary Medicine College, University of Kerbala, Kerbala, Iraq
  • Wefak Albazi Physiology Department, Veterinary Medicine College, University of Kerbala, Kerbala, Iraq
  • Muna Hussain Al-Aameli Anatomy and Histology Department, Veterinary Medicine College, University of Kerbela, Karbala, Iraq
  • Tahreer M. Al-Thuwaini Agriculture College, Al-Qasim Green University, Babil, Iraq
  • Goosoon Hadi Jhoni Family Medicine Department, College of Medicine, University of Kerbala, Kerbala, Iraq.

DOI:

https://doi.org/10.59658/jkas.v11i2.1842

Keywords:

hypercholesteremic diet, glutamate, BDNF, oxidative stress.

Abstract

An abnormality of lipid metabolism known as hypercholesteremia, which causes blood cholesterol levels to be abnormally high, is a significant health concern. This research, in light of its association with neurodegenerative disease, is of paramount importance as it aims to elucidate how elevated cholesterol levels impact glutamate levels, BDNF, Synapsin K, and ARC gene expression. Ten male rats were meticulously assigned to a control group and another group that was given a 1% cholesterol supplement for 28 days. Each group received a random assignment of rats to ensure the validity of the results. Samples of homogenized brain tissue and serum blood were obtained after the experiment's completion, following a meticulous experimental design. Triacylglycerol (TAG), low-density lipoprotein (LDL), and total cholesterol (TC) values rose significantly, whereas HDL and brain-derived neurotrophic factor (BDNF) fell sharply, according to the study's findings. The results indicated that hypercholesteremia had a negative impact on glutamate levels, BDNF, and synapses due to oxidative stress, while synapsin-1 levels in brain tissue remained unchanged. There were also notable decreases in serum and homogenized brain tissue concentrations of NO and GSH, along with increases in MDA. Additionally, there was a noticeable decrease in glutamate levels in brain tissue and down-regulation of the Activity Cytoskeleton Association protein gene (ARC gene) expression.  

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Published

06/09/2024

How to Cite

Rafaish , H. G. ., Albazi , W. ., Al-Aameli , M. H. ., Al-Thuwaini, T. M. ., & Jhoni, G. H. . (2024). The effect of an induced hypercholesteremia on the regulation of the ARC gene expression, Brain Derived Neurotropic Factor (BDNF), Synapsing-1 and neurotransmitter –glutamate in male rats. Journal of Kerbala for Agricultural Sciences, 11(2), 11–29. https://doi.org/10.59658/jkas.v11i2.1842

Issue

Vol. 11 No. 2 (2024): The second International Scientific Conference, along with its third periodic session, was held under the theme: "Applied Research and Its Role in Improving Human and Animal Health." The event took place at the College of Veterinary Medicine, University of Karbala, in May 13, 2024.

Section

Articles

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